Premium
Conjugation of nucleic acid probes to 6‐aminoglucose‐based homo‐ and copolymers. II. Application to diagnostics
Author(s) -
Delair Thierry,
Badey Bérengère,
Charles MarieHélène,
Laayoun Ali,
Domard Alain,
Pichot Christian,
Mandrand Bernard
Publication year - 1997
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/(sici)1099-1581(199709)8:9<545::aid-pat685>3.0.co;2-s
Subject(s) - copolymer , covalent bond , conjugate , reductive amination , polymer , nucleic acid , combinatorial chemistry , oligonucleotide , materials science , polymer chemistry , dna , chemistry , organic chemistry , biochemistry , catalysis , mathematical analysis , mathematics
The covalent immobilization of oligodeoxynucleotides to a 6‐deoxy‐6‐methacryloylamido‐ d ‐glucopyranose (6‐amino glucose) homopolymer and a 36% hydroxyethylmethacrylate (HEMA) copolymer was studied in order to make oligodeoxynucleotide–polymer conjugates of potential applications in diagnostics. The reducing ends of the saccharidic moieties of the (co)polymers served as reactive groups for the covalent attachment of 5′‐amino‐modified oligodeoxyribonucleotides via reductive amination. The course of the immobilization reaction was slightly affected by the polymer composition, but differences were marked as far as the aggregation of the conjugates was concerned. Evaluation of the conjugates in the quantification of hepatitis B virus DNA allowed amplification factors identical to the reference polymer. Amplification was the most efficient with conjugates obtained, at low pH, with the copolymer. © 1997 John Wiley & Sons, Ltd.