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Synthesis and Antibacterial Activity of Water‐soluble Chitin Derivatives
Author(s) -
Kim ChunHo,
Kim SoYeon,
Choi KyuSuk
Publication year - 1997
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/(sici)1099-1581(199705)8:5<319::aid-pat645>3.0.co;2-g
Subject(s) - chitin , chitosan , sodium borohydride , antibacterial activity , sodium hydroxide , hydrolysis , aqueous solution , nuclear chemistry , chemistry , escherichia coli , organic chemistry , biochemistry , bacteria , biology , gene , catalysis , genetics
Diethylaminoethyl–chitin (DEAE–chitin) was synthesized by introducing DEAE groups onto the C(6)–OH in chitin. DEAE–chitin was N‐deacetylated by heating in aqueous 10% sodium hydroxide containing sodium borohydride to prepare the diethylaminoethyl–chitosan (DEAE–chitosan). In addition, DEAE–chitin was quaternized with ethyl halide to produce triethylaminoethyl–chitin (TEAE–chitin). Their antibacterial activities were evaluated by using colony count against Staphylococcus aureus and Escherichia coli by means of shake flask method. The antibacterial activity was found to increase in order of DEAE–chitin, DEAE–chitosan, TEAE–chitin. DEAE–chitin was hydrolyzed by enzymes to investigate the effect of molecular weight on its antibacterial activities. The antibacterial activity was dependent on the structure, particularly on the molecular weight of chitin derivatives. © 1997 John Wiley & Sons, Ltd.