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Functional evaluation of hemoglobin‐and lipidheme‐vesicles as red cell substitutes
Author(s) -
Sakai Hiromi,
Hamada Kenichi,
Takeoka Shinji,
Nishide Hiroyuki,
Tsuchida Eishun
Publication year - 1996
Publication title -
polymers for advanced technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 90
eISSN - 1099-1581
pISSN - 1042-7147
DOI - 10.1002/(sici)1099-1581(199608)7:8<639::aid-pat572>3.0.co;2-2
Subject(s) - vesicle , hemoglobin , biophysics , oxygen , red blood cell , chemistry , biochemistry , biology , organic chemistry , membrane
The two kinds of red cell substitutes, hemoglobin‐vesicles (HbV) and lipidheme‐vesicles (LihV, totally synthetic oxygen carrier), were evaluated in terms of physicochemical properties such as binding and dissociating reactions of ligands (CO, O 2 and NO), rheological and structural properties. Carbonylation of Hb during the purification of Hb and the preparation of HbV is effective to prevent Hb denaturation. The rates of oxygenation of both HbV and LihV are faster than that of red blood cells (RBC). Their oxygen affinities (P 50 , HbV, 32 mmHg; LihV, 43 mmHg, cf. RBC, 28 mmHg) can be controlled to transport a sufficient amount of oxygen comparable with that of RBC. The smaller sizes of vesicles are advantageous for prompt ligand reaction and low viscosity. Both HbV and RBC show about 100 times less vasoconstrictive effects than stripped Hb. HbV shows only one sixth of the slow binding rate of NO (=endothelial derived relaxation factor) in comparison with stripped Hb. Inhibition of vasoconstriction by those vesicles is discussed from the kinetic data.