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Constituents of Croton menthodorus and their effects on electrically induced contractions of the guinea‐pig isolated ileum
Author(s) -
Capasso Anna,
Piacente Sonia,
De Tommasi Nunziatina,
Ragucci Massimo,
Pizza Cosimo
Publication year - 2000
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(200005)14:3<156::aid-ptr630>3.0.co;2-v
Subject(s) - ileum , petroleum ether , guinea pig , chemistry , ether , biological activity , pharmacognosy , croton , chromatography , stereochemistry , biochemistry , biology , in vitro , organic chemistry , endocrinology , extraction (chemistry)
The present study examines the effects of the extracts [petroleum ether, CHCl 3 , CHCl 3 MeOH (9:1) and MeOH], partially purified fractions and pure compounds from Croton menthodorus on the electrically induced contractions of the isolated guinea‐pig ileum (ECI). The results of the experiments indicate that CHCl 3 /MeOH (9:1) and MeOH extracts, tested at concentrations of 100, 50 and 25 µg/mL, dose‐dependently reduced the guinea‐pig ileum contractions, whereas petroleum ether and CHCl 3 extracts did not affect it. Furthermore, the partially purified fractions III–VI from the CHCl 3 /MeOH extract, each tested at concentrations of 100, 50 and 25 µg/mL also inhibited ECI. Finally, pure compound 1 (6 × 10 −6 , 3 × 10 −6 , 1 × 10 −6 M ) isolated and purified from the most active fraction III significantly reduced, in a dose‐dependent manner, the electrical contractions of the ileum. Compound 1 was identified by NMR and EI‐MS data as the morphinandien‐7‐one, O‐methylflavinantine. Copyright © 2000 John Wiley & Sons, Ltd.