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Acute and subchronic evaluation of Indigofera arrecta : absence of both toxicity and modulation of selected cytochrome P450 isozymes in ddY mice
Author(s) -
Nyarko Alexander K.,
Ankrah NiiAyi,
Ofosuhene Mark,
Sittie Archibald A.
Publication year - 1999
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199912)13:8<686::aid-ptr519>3.0.co;2-b
Subject(s) - glutathione , cytochrome p450 , isozyme , acute toxicity , in vivo , pharmacology , pentobarbital , toxicity , medicine , endocrinology , biology , metabolism , chemistry , enzyme , biochemistry , microbiology and biotechnology
Indigofera arrecta , an anti diabetic plant was investigated in ddY mice to determine its acute and subchronic effects, and whether it modulated hepatic cytochrome P450 (CYP) isozymes and glutathione (GSH). No mortality was observed in the acute (up to 10 g I. arrecta /kg body wt, p.o.) and subchronic (2 g I. arrecta /kg body wt, p.o. daily for 30 days) studies. The extract did not alter haematological indices, serum and tissue lipids and glutathione but lowered serum bile acids. The latter phenomenon is under further investigation. Neither the duration of pentobarbital (PB) and zoxazolamine (ZA) effects in vivo , nor CYP‐dependent 7‐ethoxyresorufin‐O‐deethylase (EROD), 7‐pentoxyresorufin‐O‐depentylase (PROD) and p‐nitrophenol hydroxylase (PNPH) activities in vitro were altered by I. arrecta . The extract was thus devoid of overt acute and subchronic toxic effects, and did not affect CYPs and GSH whose modulation may cause interactions of components in a multiple drug therapy. Copyright © 1999 John Wiley & Sons, Ltd.