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Antiplasmodial activity of selected Sudanese medicinal plants with emphasis on Acacia nilotica
Author(s) -
ElTahir Ahmed,
Satti Gwiria M. H.,
Khalid Sami A.
Publication year - 1999
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199909)13:6<474::aid-ptr482>3.0.co;2-6
Subject(s) - traditional medicine , phytochemical , terpenoid , biology , gardenia , cassia , pharmacognosy , bioassay , plasmodium falciparum , medicinal plants , botany , biological activity , in vitro , medicine , malaria , biochemistry , traditional chinese medicine , alternative medicine , genetics , pathology , immunology
Twenty‐two plant organs from eleven plants comprising five families were extracted and screened for antiplasmodial activity in vitro against Plasmodium falciparum 3D7 (chloroquine sensitive) and Dd2 (chloroquine resistant and pyrimethamine sensitive). Fifty nine percent of plant extracts from 22 extracts exerted activity on P. falciparum strain 3D7 with an IC 50 less than 50 µg/mL, whereas 43% of plant extracts showed an IC 50 value within 50 µg/mL on Dd2 strains. Plant extracts from Gardenia lutea, Haplophyllum tuberculatum, Cassia tora, Acacia nilotica and Aristolochia bracteolata possessed IC 50 values less than 5 µg/mL on both tested strains. Bioassay guided fractionation of A. nilotica revealed that the ethyl acetate extract possessed the highest activity (IC 50 = 1.5 µg/mL). Fraction 2 ( R f = 0.75) prepared by preparative chromatography showed the highest activity on P. falciparum (IC 50 = 1.7 µg/mL). Phytochemical analysis indicated that the most active phase contained terpenoids and tannins and was devoid of alkaloids and saponins. The effect of plant extracts on lymphocyte proliferation showed low toxicity to the human cells. This plant has been subjected to long term clinical trials in folk medicine and is a promising plant. Copyright © 1999 John Wiley & Sons, Ltd.