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Relationship between the antidiarrhoeal effects of Hange‐Shashin‐To and its active components
Author(s) -
Kase Yoshio,
Saitoh Kazuko,
Makino Bunsyo,
Hashimoto Kazunori,
Ishige Atsushi,
Komatsu Yasuhiro
Publication year - 1999
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199909)13:6<468::aid-ptr504>3.0.co;2-v
Subject(s) - antidiarrhoeal , ginseng , traditional medicine , glycyrrhizin , oral administration , pharmacology , radix (gastropod) , medicine , chemistry , chromatography , castor oil , biochemistry , botany , biology , alternative medicine , pathology
This study was designed to examine the relationship between the antidiarrhoeal effects of Hange‐Shashin‐To (TJ‐14) and its active components. Oral treatment with TJ‐14 at 1000 mg/kg significantly inhibited castor oil‐induced diarrhoea. Both the 50% methanol eluate fraction (fraction III) and the methanol eluate fraction (fraction IV) showed antidiarrhoeal effects at oral doses of 68 mg/kg and 63 mg/kg, respectively, corresponding to 1000 mg/kg of TJ‐14. TJ‐14 (1000 mg/kg, p.o.) showed a significant increase in blood corticosterone levels. Increased blood corticosterone was noted after the oral administration of 63 mg/kg of fraction IV. The inhibitory activity of TJ‐14 on cyclooxygenase‐2 (COX‐2) was also observed in fractions III and IV. The main component of fraction III was Scutellariae Radix ‐derived baicalin. Fraction IV contained Glycyrrhizae Radix ‐derived glycyrrhizin and isoliquiritin, Coptidis Rhizoma ‐derived berberine, coptisine and palmitine. Ginseng Radix ‐derived saponins were also present in fraction IV. These compounds inhibited castor‐oil induced diarrhoea at oral doses of 10 or 30 mg/kg. Thus, the present results indicate that Scutellariae Radix, Glycyrrhizae Radix, Ginseng radix and Coptidis Rhizoma ‐derived components are involved in the antidiarrhoeal action of TJ‐14. Copyright © 1999 John Wiley & Sons, Ltd.