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Effects of Daniellia oliveri bark on isolated rat bladder
Author(s) -
Onwukaeme N. D.,
Lot T. Y.,
Udoh F. V.
Publication year - 1999
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199908/09)13:5<416::aid-ptr469>3.0.co;2-r
Subject(s) - glycoside , chemistry , bark (sound) , butanol , acetone , cardiac glycoside , traditional medicine , pharmacognosy , residue (chemistry) , muscarinic acetylcholine receptor , stereochemistry , receptor , biological activity , biochemistry , in vitro , ethanol , biology , medicine , organic chemistry , ecology , ouabain , sodium
The stem bark of Daniellia oliveri was screened phytochemically and a methanol extract prepared.Condensed tannins, saponins, cyanogenetic and cardiac glycosides were identified in the crude drug. The cardiac glycoside components in the methanol extract were precipitated with acetone to yield a reddish‐brown residue. The n ‐butanol soluble fraction of an aqueous solution of this residue tested positive for cardiac glycosides and was shown by TLC to contain steroidal compounds. This fraction was subjected to pharmacological studies on isolated rat bladder smooth muscle. It had no effect on purinergic neurotransmission but was a noncompetitive antagonist for muscarinic receptors. Copyright © 1999 JohnWiley & Sons, Ltd.