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Inhibition of keratinocyte growth by different Nepalese Zanthoxylum species
Author(s) -
Kumar Sunil,
Müller Klaus
Publication year - 1999
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199905)13:3<214::aid-ptr431>3.0.co;2-2
Subject(s) - hacat , ic50 , lactate dehydrogenase , keratinocyte , traditional medicine , cytotoxicity , chemistry , pharmacognosy , membrane , positive control , cell growth , biological activity , biochemistry , stereochemistry , in vitro , enzyme , medicine
A total of 11 methanol extracts obtained from four different Nepalese Zanthoxylum species were screened for their antiproliferative activity against the growth of human keratinocytes (HaCaT cells). The extract obtained from Z. armatum barks was highly active with an IC 50 value of 11 µg/mL. Also, the extracts obtained from Z. oxyphyllum barks and roots with IC 50 values of 53 and 57 µg/mL, respectively, showed potent activity. Their antiproliferative activity was not due to cytotoxic effects on cell membranes, as documented by the activity of lactate dehydrogenase released from the cytoplasm of keratinocytes, which did not exceed that of the control value. Rather, they also protected against radical‐induced damage to model membranes stimulated with 2,2′‐azo‐bis(2‐amidinopropane) dihydrochloride. Copyright © 1999 John Wiley & Sons, Ltd.