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Inhibition by chlorophyllin of forward and reverse bacterial mutagenicity of benzo[a]pyrene‐7,8‐dihydrodiol‐9,10‐epoxide
Author(s) -
Surh YoungJoon
Publication year - 1998
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199812)12:8<580::aid-ptr364>3.0.co;2-f
Subject(s) - chlorophyllin , chemistry , mutagen , epoxide , ames test , cytotoxicity , benzo(a)pyrene , pyrene , salmonella , carcinogen , biochemistry , in vitro , stereochemistry , chlorophyll , organic chemistry , bacteria , biology , genetics , catalysis
Chlorophyllin (CHL), a water soluble derivative of the green pigment chlorophyll, was evaluated for its antimutagenic activity towards benzo[a]pyrene‐7,8‐dihydrodiol‐9,10‐epoxide (BPDE). CHL suppressed dose‐dependently the formation of 8‐azaguanine‐resistant mutant colonies in Salmonella typhimurium TM677 treated with BPDE. Under the same experimental conditions, the cytotoxicity of this direct‐acting mutagen was concomitantly blunted by CHL. The protective effect of CHL against BPDE mutagenicity was confirmed in the Salmonella histidine + reversion assay CHL also reduced the in vitro covalent binding of BPDE to DNA. Copyright © 1998 John Wiley & Sons, Ltd.