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Cytotoxic and antitumour activity from Bursera fagaroides ethanol extract in mice with L5178Y lymphoma
Author(s) -
PueblaPérez Ana María,
HuacujaRuiz Luis,
RodríguezOrozco Gabriela,
VillaseñorGarcía María Martha,
MirandaBeltrán María de la Luz,
Celis Alfredo,
SandovalRamírez Lucila
Publication year - 1998
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199812)12:8<545::aid-ptr349>3.0.co;2-s
Subject(s) - cytotoxic t cell , lymphoma , medicine , pharmacology , ed50 , oral administration , ethanol , carcinoma , traditional medicine , immunology , in vitro , biology , biochemistry , receptor
Chloroform extracts of Bursera fagaroides (Burseracea) have previously shown antitumour activity against the Walker carcinoma 256 tumour system (WA16). In the present work we have determined the cytotoxic and antitumour activity of the ethanol extract (70%) of the bark of B. fagaroides using the L5178Y lymphoma. The cytotoxic activity is expressed as the ED 50 of the L5178Y lymphoma cells in culture, (20 µg/mL) whilst the antitumour activity was shown via a tumour growing inhibition test, measuring survival of BALB/c mice (2 × 10 4 cells L5178Y i.p.). 24 h after inoculation mice were treated with 50 or 100 mg/kg of extract daily, over 15 days in independent groups of 10, using two administration routes. We observed the tumour evolution with and without treatment. Oral administration resulted in 8% of mice being tumour free after 60 day whilst intraperitoneal administration showed 26% survived at a dose of 100 mg/kg/day for 15 days. A significant increase in the survival of the treated animals (at 50 mg/kg/day over 15 days) was found compared with those treated with placebo or without treatment. Copyright © 1998 John Wiley & Sons, Ltd.