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Extract of gravel root (rhizome of Eupatorium purpureum ) inhibits integrin‐dependent U937 cell adhesion
Author(s) -
Habtemariam Solomon
Publication year - 1998
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199809)12:6<422::aid-ptr333>3.0.co;2-q
Subject(s) - u937 cell , fibronectin , adhesion , cell adhesion , rhizome , chemistry , microbiology and biotechnology , extracellular matrix , biology , biochemistry , cell , botany , in vitro , organic chemistry
The effect of the antirheumatic herbal drug, gravel root (rhizome of Eupatorium purpureum ), was investigated on integrin‐dependent U937 cell adhesion to endothelial cells and extracellular matrix protein, fibronectin. In the presence of gravel root extract (6.3–200 μg/mL), U937 cells lowered their capacity for phorbol myristate acetate (PMA)‐mediated adhesion to tumour necrosis factor‐α (TNF)‐activated endothelial cells or ICAM‐1 coated plates. Similarly, the PMA mediated, LFA‐1‐dependent, homotypic cell aggregation in U937 cells was potently inhibited by gravel root extract. The α 4 β 1 and α 5 β 1 ‐mediated adhesion of unstimulated U937 cells to fibronectin coated plates was also inhibited by gravel root extract. Neither the TNF‐induced enhancement of expression of ICAM‐1 on endothelial cell surface nor the TNF‐induced endothelial adhesiveness to U937 cells was, however, inhibited by gravel root extract. Since all concentrations of gravel root extract tested failed to alter the proliferation of U937 cells, the observed activity was unlikely to be due to nonspecific suppression of cells. © 1998 John Wiley & Sons, Ltd.