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Effect of sesamin on growth and arachidonic acid content of neoplastic and non‐neoplastic prostate epithelial cell cultures
Author(s) -
Griffiths Gareth,
Jones Helen E.,
Eaton Colby L.,
Shimizu Sakayu,
Stobart A. Keith
Publication year - 1998
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199809)12:6<417::aid-ptr334>3.0.co;2-9
Subject(s) - sesamin , neoplastic transformation , lignan , arachidonic acid , neoplastic cell , cell culture , biology , biochemistry , linolenic acid , cell , fatty acid , chemistry , linoleic acid , food science , genetics , gene , carcinogenesis , enzyme , botany
Sesamin (a lignan present in sesame oil) is reported to specifically inhibit Δ5 desaturation and the formation of arachidonic acid (20:4n‐6) from dihomogamma‐linolenic acid (20:3n‐6) in cell free extracts. In this study the effects of sesamin have been evaluated on cell cultures derived from canine prostatic tissues of epithelial origin from neoplastic and non‐neoplastic tissues. Sesamin reduced cell proliferation in a dose dependent manner in both cell lines with TD 50 values of 91 ± 16 μ M (non‐neoplastic) and 132 ± 12 μ M (neoplastic). In the non‐neoplastic cells, supplemented with gamma‐linolenic acid (18:3n‐6) in the culture media, sesamin had little effect on the content of C16 and C18 fatty acids but markedly reduced the content of 20:4n‐6 and caused an accumulation of 20:3n‐6. This observation is consistent with sesamin inhibiting Δ5 desaturase activity. Sesamin, however, had little effect on the content and ratio of 20:3n‐6 and 20:4n‐6 fatty acids in neoplastic cells indicating that Δ5 desaturase activity was low in these cells. Thus, sesamin is an effective compound for reducing Δ5 desaturase activity in non‐neoplastic cell cultures and could be used to manipulate the content of series 1 and 2 prostaglandins derived from 20:3n‐6 and 20:4n‐6, respectively. © 1998 John Wiley & Sons, Ltd.