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Insulinotropic activity of Tinospora crispa extract: effect on ß‐cell Ca 2+ handling
Author(s) -
Noor Hamdan,
Ashcroft Stephen J. H.
Publication year - 1998
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199803)12:2<98::aid-ptr195>3.0.co;2-f
Subject(s) - extracellular , intracellular , insulin , secretagogue , cytosol , mechanism of action , pharmacology , stimulation , chemistry , in vitro , biological activity , efflux , biochemistry , biology , endocrinology , enzyme
Abstract The mechanism of insulinotropic action of Tinospora crispa was investigated in vitro using an insulin‐secreting clonal ß‐cell line, HIT‐T15. The aqueous extract sensitizes the ß‐cell to extracellular Ca 2+ and promotes intracellular Ca 2+ accumulation which in turn causes increased insulin release. The increase in cytosolic Ca 2+ concentration is due to stimulation of Ca 2+ uptake from the extracellular medium and inhibition of Ca 2+ efflux from the cytosol. That the mechanism of insulinotropic action of T. crispa is physiological suggests that the insulin secretagogue/s present in the extract could indeed be a potential source of a specific oral hypoglycaemic agent for the treatment of non‐insulin‐dependent diabetes mellitus. © 1998 John Wiley & Sons, Ltd.