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Effect of HD‐03, a herbal formulation, on the antioxidant defence system in rats
Author(s) -
Mitra S. K.,
Venkataranganna M. V.,
Sundaram R.,
Gopumadhavan S.
Publication year - 1998
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199803)12:2<114::aid-ptr206>3.0.co;2-8
Subject(s) - lipid peroxidation , antioxidant , glycogen , chemistry , ccl4 , pharmacology , biochemistry , glycogenolysis , medicine , endocrinology , carbon tetrachloride , organic chemistry
The effect of HD‐03, a herbal formulation was investigated on the hepatocellular antioxidant defence system in CCl 4 treated rats. Three doses of CCl 4 were administered orally with liquid paraffin (1:1) to induce hepatic damage. Twenty four hours after the last dose, blood was collected by decapitation for the estimation of serum ALT and AST. The levels of antioxidant enzymes, lipid peroxidation and glycogen in the liver were estimated. Nineteen days pretreatment with HD‐03 (750 mg/kg) significantly reversed CCl 4 ‐induced changes in serum ALT and AST levels. HD‐03 pretreatment also significantly reversed the CCl 4 ‐induced changes in the different components of the cellular antioxidant system and lipid peroxidation. Pretreatment with HD‐03 significantly restored the hepatic glycogen levels which were depleted in CCl 4 intoxicated rats. The observed reversal produced by HD‐03 in the serum AST and ALT may be due to the membrane stabilizing potential which helps in preventing the leakage of intracellular enzymes into the systemic circulation. The increase in the hepatic glycogen levels in HD‐03 pretreated rats, indicates its preventive effect on subcellular injury caused by CCl 4 , which leads to glycogenolysis, as a result of disturbance in the intracellular Ca +2 pool. The inhibition of lipid peroxidation and enhancement in the activity of antioxidant enzymes by HD‐03 may be due to the direct free radical scavenging activity and reactivation of these enzymes in the liver. Thus the antioxidant potential and inhibitory effect onlipid peroxidation may play an important role in the antihepatotoxic activity of HD‐03. © 1998 John Wiley & Sons, Ltd.