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The influence of extracts of Tricholomopsis rutilans (schff.ex fr.) sing. on the binding of LPS to CD14 + ‐cells and on the release of inflammatory mediators
Author(s) -
Koch J.,
Witt S.,
Liberra K.,
Lindequist U.
Publication year - 1998
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(1998)12:1+<s27::aid-ptr240>3.0.co;2-6
Subject(s) - pharmacognosy , cd14 , chemistry , pharmacology , traditional medicine , biochemistry , biology , medicine , biological activity , in vitro , receptor
Abstract Looking for new pharmacologically active agents or lead structures, pharmaceutical chemists and biologists have traditionally dealt with secondary products from terrestrial plants and animals. During the past few years fungi have become more and more integrated into different screening systems. Antibacterial and immune response modifying compounds from fungi are known (Lindequist et al., 1990). Various basidiomycetes extracts were tested for their ability to inhibit the binding of lipopolysaccharide (endo‐toxin, LPS) to the endotoxin receptor CD14 and their influence on the release of mediators such as interleukin 1 (IL‐1), interleukin 6 (IL‐6) and tumour necrosis factor α (TNF‐α). We tested the ethanol extracts of the whole fungi as well as extracts of the skin and the fruiting bodies and cultivated mycelium. The ethanol extracts of Tricholomopsis rutilans reduced the binding of LPS and the release of mediators. In a concentration of 100 μg/mL there was an inhibition by more than a half of the LPS binding. Furthermore we found a significant decrease in the LPS‐induced release of IL‐1 and TNF‐α in a concentration dependent manner. The skin extract of T. rutilans was selected for further examination and isolation of active compounds. © 1998 John Wiley & Sons, Ltd.