Premium
Characterization of an extract from the leaves of Cissampelos sympodialis Eichl. on the spontaneous tone of isolated trachea
Author(s) -
Thomas George,
Burns Fiona,
Pyne Susan,
Pyne Nigel J.
Publication year - 1997
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199711)11:7<496::aid-ptr137>3.0.co;2-7
Subject(s) - guinea pig , smooth muscle , phosphodiesterase , cyclic nucleotide phosphodiesterase , ic50 , chemistry , ethanol , in vitro , biochemistry , biology , endocrinology , enzyme
An aqueous fraction of the ethanol extract of the leaves (AFL) of Cissampelos sympodialis Eichl. (Menispermaceae) was shown to relax the spontaneous tone of guinea‐pig tracheal smooth muscle rings with an IC 50 of 20.5 μg/mL. The AFL inhibited cyclic nucleotide phosphodiesterase activity in isolated smooth muscle homogenates and stimulated an increase in intracellular cAMP synthesis in intact cultured smooth muscle cells. A complete inhibition of phosphodiesterase activity in isolated homogenates was almost achieved. These studies indicate that the AFL contains a substance which is a non‐selective, but highly potent inhibitor of phosphodiesterase activity. This probably accounts for its effect on the spontaneous tone of guinea‐pig tracheal smooth muscle. © 1997 John Wiley & Sons, Ltd.