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Effects of baicalein isolated from roots of Scutellaria baicalensis Georgi on interleukin 1β‐ and tumour necrosis factor α‐induced tissue‐type plasminogen activator and plasminogen activator inhibitor‐1 production in cultured human umbilical vein endothelial cells
Author(s) -
Kimura Yoshiyuki,
Okuda Hiromichi,
Yokoi Hayashi Kumi,
Matsushita Nobutoshi
Publication year - 1997
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199708)11:5<363::aid-ptr106>3.0.co;2-u
Subject(s) - umbilical vein , scutellaria baicalensis , plasminogen activator , baicalein , tumor necrosis factor alpha , tissue plasminogen activator , cytokine , t plasminogen activator , interleukin 8 , biology , interleukin , plasminogen activator inhibitor 1 , endocrinology , protein kinase c , medicine , chemistry , microbiology and biotechnology , pharmacology , kinase , biochemistry , in vitro , pathology , alternative medicine , traditional chinese medicine
The effects were examined of baicalein on tissue‐type plasminogen activator (t‐PA) and plasminogen activator inhibitor‐1 (PAI‐1) production stimulated by interleukin‐1α (IL‐1β) and tumour necrosis factor‐α (TNF‐α) in cultured human vein umbilical endothelial cells (HUVECs). IL‐1β and TNF‐α increased PAI‐1 production, but they decreased t‐PA production. Baicalein isolated from the roots of Scutellaria baicalensis Georgi, inhibited the PAI‐1 production stimulated by IL‐1β and TNF‐α, but it had no effect on the t‐PA. In addition, protein kinase C (PKC) inhibitors such as H7 and sphingosine decreased the reduction of t‐PA and the increase of PAI‐1 by IL‐1β and TNF‐α. On the other hand, dibutryl cAMP and forskolin had no effect on the t‐PA reduction and PAI‐1 increase by IL‐1β and TNF‐α. Moreover, IL‐1β and TNF‐α did not cause [Ca 2+ ] elevation. These results suggest that the inhibitory action of baicalein on the PAI‐1 production by both cytokines might be directly mediated through PKC inhibition. © 1997 John Wiley & Sons, Ltd.