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The effects of capsaicin on the metabolic activation of heterocyclic amines and on cytochrome P450 1A2 activity in hamster liver microsomes
Author(s) -
Teel Robert W.,
Zhang Zhuo,
Huynh Huong,
Hamilton Solomon
Publication year - 1997
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199708)11:5<358::aid-ptr102>3.0.co;2-s
Subject(s) - capsaicin , microsome , hamster , cytochrome p450 , chemistry , pharmacology , monooxygenase , cytochrome , biochemistry , enzyme , biology , endocrinology , receptor
Capsaicin, the principal component of Capsicum fruits, strongly inhibited the in vitro mutagenic activity of five heterocyclic amines (HCAs) in Salmonella typhimurium strain TA98 by hamster liver microsomes. This correlated with the inhibition of microsome associated demethylation of methoxyresorufin (MROD) by capsaicin. MROD activity is mediated by cytochrome P450 1A2 and CYP450 1A2 mediates the metabolic activation of HCAs. Enzyme kinetics studies indicated a noncompetitive type of inhibition by capsaicin. Liver microsomes prepared from hamsters 6 h and 24 h after administration of a single oral dose of capsaicin in olive oil at either 2 mg/kg or 10 mg/kg body weight expressed a small but statistically significant inhibition of MROD activity compared with microsomes from control (untreated) animals. These results suggest that capsaicin can modulate the activity of cytochrome P450 1A2. © 1997 John Wiley & Sons, Ltd.