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Neuroprotective Effects of Toki‐Shakuyaku‐San (TJ‐23) on Glutamate Induced Neuronal Death in Cultured Cerebellar Granule Cells
Author(s) -
Zhang Xiangqian,
Hagino Nobuyoshi,
Nozaki Takehiro
Publication year - 1997
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199703)11:2<107::aid-ptr942>3.0.co;2-s
Subject(s) - neuroprotection , glutamate receptor , granule (geology) , glutamic acid , programmed cell death , cerebellum , chemistry , biology , neuroscience , endocrinology , pharmacology , medicine , biochemistry , apoptosis , amino acid , paleontology , receptor
The neuroprotective properties of Toki‐Shakuyaku‐San (TJ‐23) were investigated in L‐glutamic acid (Glu)‐induced neuronal death in 8 day primary cultured cerebellar granule cells. After a 4 h incubation in Mg 2+ ‐free Locke's solution at 37°C, the neuronal survival was 88.5%. An application of 100 μ M of Glu into Mg 2+ ‐free Locke's solution resulted in 40% neuronal survival. Further application of either 0.05 or 0.5 mg/mL of TJ‐23 into the above solution resulted in 65% and 71% of neuronal survival, respectively. In order to investigate the involvement of ions in the active mechanism of TJ‐23, we examined Glu‐induced neuronal death in the presence or absence of NaCl, Ca 2+ , and Mg 2+ . It was observed that TJ‐23 selectively inhibited the Ca 2+ ‐dependent neuronal death. From these observations, it is inferred that TJ‐23 has a neuroprotective effect on Glu‐induced neuronal death. © 1997 by John Wiley & Sons, Ltd.