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Inhibitory Effect of (−)‐Epicatechin 4‐Benzylthioether on the Growth of Glioma Cells in Culture
Author(s) -
Hsu Stephen ShihFan,
Jou ShuoBin,
Hsu FengLin,
Yang TzuFeng,
Cheng JueiTang
Publication year - 1996
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199612)10:8<643::aid-ptr924>3.0.co;2-1
Subject(s) - glioma , thymidine , glutathione , biochemistry , intracellular , glutathione reductase , glutathione peroxidase , growth inhibition , extracellular , enzyme , peroxidase , chemistry , cell culture , cell growth , inhibitory postsynaptic potential , microbiology and biotechnology , dna , biology , cancer research , genetics , neuroscience
The effect of (−)‐epicatechin 4‐benzylthioether (EC‐BE) on the growth of cultured rat glioma cells was investigated. EC‐BE was found to produce a concentration‐dependent inhibitory effect on the growth of glioma cells. Inhibition was also observed in samples treated with (−)‐epicatechin acetoxymethyl ester (EC‐AM). The original compound (−)‐epicatechin, which gave rise to EC‐AM and EC‐BE, failed to produce a similar result. Because both EC‐BE and EC‐AM are membrane permeable, while (−)‐epicatechin is not, the results indicated that the (−)‐epicatechin and its derivatives need to be intracellular in order to produce an effect. Moreover, it is demonstrated that EC‐BE inhibited the incorporation of [ 3 H]thymidine into DNA while the uptake of extracellular [ 3 H]thymidine by glioma cells was not affected. The activities of redox enzymes such as glutathione peroxidase and glutathione reductase were attenuated in cells treated with EC‐BE. These data suggest that EC‐BE can inhibit the growth of glioma cells by affecting a redox pathway related to the formation of DNA.