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Inhibitory Effect of Poria cocos on 12‐ O ‐Tetradecanoylphorbol‐13‐Acetate‐Induced Ear Oedema and Tumour Promotion in Mouse Skin
Author(s) -
Kaminaga Tomohiro,
Yasukawa Ken,
Takido Michio,
Tai Takaaki,
Nunoura Yoshiki
Publication year - 1996
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199611)10:7<581::aid-ptr907>3.0.co;2-3
Subject(s) - triterpene , 12 o tetradecanoylphorbol 13 acetate , polyporaceae , tumor promotion , sclerotium , tetradecanoylphorbol acetate , chemistry , pharmacognosy , anti inflammatory , biochemistry , pharmacology , traditional medicine , medicine , biology , phorbol ester , biological activity , carcinogenesis , in vitro , botany , enzyme , pathology , protein kinase c , gene , alternative medicine
Abstract The methanol extract from the sclerotium of Poria cocos was found to inhibit 12‐ O ‐tetradecanoylphorbol‐13‐acetate (TPA)‐induced tumour promotion in two‐stage carcinogenesis in mouse skin. From the active fraction of the extract, eight lanostane‐type triterpene acids and four 3,4‐secolanostane‐type triterpene acids were isolated. The isolated compounds showed inhibitory activity against TPA‐induced ear inflammatory oedema. The 50% inhibitory dose of pachymic acid, 3‐ O ‐acetyl‐16α‐hydroxytrametenolic acid, dehydropachymic acid, dehydroeburiconic acid, 3β‐hydroxylanosta‐7,9(11),24‐trien‐21‐oic acid, poricoic acid A and poricoic acid B for TPA‐induced inflammation was 17–44 μg/ear, at a grade corresponding to that of hydrocortisone.