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The Immunosuppressive Effects of Globularia alypum Extracts
Author(s) -
Fehri B.,
Tebbett I. R.,
Freiburger B.,
Karlix J.
Publication year - 1996
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199609)10:6<539::aid-ptr898>3.0.co;2-a
Subject(s) - incubation , ic50 , ethanol , chemistry , in vitro , cytotoxicity , microbiology and biotechnology , stimulation , cell culture , mixed lymphocyte reaction , chromatography , pharmacology , biochemistry , t cell , biology , immunology , immune system , endocrinology , genetics
Responder lymphocytes were cultured with an equal number of irradiated stimulator lymphocytes from another donor for 6 days in RPMI 1640 (a modified Mc Coh's 5 A Medium). After 48 h incubation with PMA (phorbol 12‐myristate), PHA (phytohaematogglutin) and 120 h with MLR (mixed lymphocytes reaction), the cells were labelled with 3 H‐thymidine. Cells were harvested in a scintillation counter Globularia alypum L. extracts were dissolved in HPLC quality ethanol or water and diluted in RPMI to concentrations ranging from 0.156–10 μg/mL. Extract solutions were added immediately after cell stimulation in the cell walls. Cyclosporin A was used as a control. Both the water and ethanol extracts of Globularia alypum L. demonstrated a dose response effect in all three systems of MLR, PHA and PMA with no statistical difference in water or ethanol extracts. The IC 50 were: PHA 1.14 μg/mL, PMA 1.05 μg/mL and MLR 2.06 μg/mL. The PHA and MLR IC 50 are approximately double the IC 50 for cyclosporin A (0.5 μg/mL). The effects of Glubularia alypum L. in PMA stimulated lymphocytes suggests it may suppress T cell function through a pathway that cyclosporin A does not effect.