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Radical Scavenging Evaluation of Green Tea Extracts
Author(s) -
Fourneau C.,
Laurens A.,
Hocquemiller R.,
Cavé A.
Publication year - 1996
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199609)10:6<529::aid-ptr887>3.0.co;2-f
Subject(s) - dpph , chemistry , rutin , catechin , scavenging , fractionation , gallate , antioxidant , polyphenol , epigallocatechin gallate , epicatechin gallate , bioassay , chromatography , organic chemistry , high performance liquid chromatography , food science , nuclear chemistry , biology , genetics
Abstract The free radical scavenging activity of Thea sinensis leaf extract was evaluated with a chemical test involving diphenylpicrylhydrazyl radical (DPPH). The hot aqueous green tea extract demonstrated antiradical activity comparable with rutin and vitamin E used as a standard. Liquid–liquid fractionation monitored by scavenging radical bioassay followed by preparative high pressure liquid chromatography led to catechin derivatives. The antiradical activity of the main component of tea leaves was identified as epigallocatechin gallate the major component demonstrating activity.

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