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Confirmatory in vivo Electrodiagnostic and Electromyographic Studies for Neuromuscular Junctional Blocking Action of Cleistanthus collinus Leaf Extract in Rat
Author(s) -
Vijayalakshmi K. M.,
Kumar N. V. Nanda,
Pagala M. K. D.
Publication year - 1996
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199605)10:3<215::aid-ptr816>3.0.co;2-n
Subject(s) - neuromuscular junction , electromyography , medicine , blocking (statistics) , in vivo , pharmacology , neuroscience , biology , physical medicine and rehabilitation , computer science , computer network , microbiology and biotechnology
Detailed electromyographic and electrodiagnostic studies were made on sciatic nerve–anterior tibialis muscle preparations in rat in vivo for the first time to confirm the neuromuscular junctional (NMJ) blocking action of Cleistanthus collinus leaf extract (CCLE) and the transient reversal of NMJ blockade by neostigmine. A sublethal dose of 175 mg dry leaf powder/kg body weight of rat caused NMJ blockade confirmed by a decremental response in nerve evoked compound muscle action potentials (NCMAPs). The study suggests the action of the toxic plant extract on acetylcholine (ACh) receptor sites. Muscle evoked compound muscle action potentials (MCMAPs) showed negligible decremental response suggesting the action at the NMJ level only and not on muscle excitation and contractility. The CCLE poisoning revealed a specific diagnostic EMG pattern which might be of help to clinicians in diagnosing Cleistanthus poisoning. The studies confirm that the leaf extract contains a proven and promising new NMJ blocker.