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Inhibition of Avian Myeloblastosis Virus Reverse Transcriptase by an Alkaloid Purified from Peschiera campestris
Author(s) -
Jucá M. B.,
Aoyama H.
Publication year - 1996
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199603)10:2<113::aid-ptr781>3.0.co;2-b
Subject(s) - reverse transcriptase , alkaloid , virology , biology , virus , chemistry , stereochemistry , biochemistry , rna , gene
12‐methoxy‐Nb‐methylvoachalotine, an alkaloid purified from Peschiera campestris inhibited avian myeloblastosis virus reverse transcriptase activities. This inhibition was dependent on the template‐primer used; in comparison with polyriboadenylic acid, the polyribocytidylic acid‐directed reaction was more affected by the alkaloid. A similar inhibition by the alkaloid was observed both in the presence of polyriboadenylic acid or its 2′‐fluorinated analogue. This inhibition also occurred in the presence of Mn 2+ instead of Mg 2+ as the divalent cation. Classical non‐competitive inhibition kinetics were obtained with respect to activated DNA and to polyriboadenylic acid.