Premium
Effects of alkaloids from Corydalis decumbens on contraction and electrophysiology of cardiac myocytes
Author(s) -
Kadota Shigetoshi,
Sun XiaoLi,
Basnet Purusotam,
Namba Tsuneo,
Momose Yasunori
Publication year - 1996
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/(sici)1099-1573(199602)10:1<18::aid-ptr765>3.0.co;2-k
Subject(s) - bullfrog , protopine , electrophysiology , atrium (architecture) , myocyte , isoquinoline , contraction (grammar) , chemistry , voltage clamp , patch clamp , ventricle , corydalis , alkaloid , biophysics , pharmacology , medicine , biology , stereochemistry , endocrinology , alternative medicine , traditional chinese medicine , pathology , atrial fibrillation
The chloroform extract of Corydalis decumbens significantly increased the beating amplitude of cultured myocardial cell sheets. Chemical analysis led to the isolation of isoquinoline and protopine alkaloids. Of these isolated alkaloids, corlumidine and (+)‐adlumidine increased the beating amplitude, but (+)‐egenine decreased the beating rate and beating amplitude while protopine did not show any activity. We also studied the effects of (+)‐egenine and corlumidine on contractile responses and Ca 2+ currents in single bullfrog atrial cells using the voltage‐clamp method. (+)‐Egenine inhibited Ca 2+ current by 68% of the control in single cell of bullfrog atrium, while corlumidine increased Ca 2+ current to 60% at a concentration of 0.03 mM.