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In vivo synthesis and purification of radioactive p ‐hydroxybenzylglucosinolate in Sinapis alba L.
Author(s) -
Chen Sixue,
Halkier Barbara Ann
Publication year - 2000
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(200005/06)11:3<174::aid-pca513>3.0.co;2-q
Subject(s) - chemistry , glucosinolate , myrosinase , in vivo , biochemistry , tyrosine , biosynthesis , de novo synthesis , sinapis , chromatography , botany , enzyme , microbiology and biotechnology , biology , brassica
Glucosinolates are amino acid‐derived, natural plant products found in the order Capparales. Radiolabelled glucosinolates for use in biological studies concerning metabolism and transport are not commercially available. We have developed a method for the synthesis and isolation of radiolabelled p ‐hydroxybenzylglucosinolate ( p ‐OHBG) produced in vivo by Sinapis alba , which has the tyrosine‐derived p ‐OHBG as its major glucosinolate. The method is based on administration of radiolabelled tyrosine to jasmonate‐treated primary leaves, which have high de novo biosynthesis of p ‐OHBG. After incubation, the plant material was extracted with methanol and the glucosinolates purified by a procedure including two ion exchange chromatographic steps. The method provides an attractive alternative to chemical synthesis of radiolabelled glucosinolates and is applicable to other plants producing a single or one major glucosinolate. Copyright © 2000 John Wiley & Sons, Ltd.