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Analysis of cucurbitacins in medicinal plants by high‐<?tcaps=1>p<?tcaps=0>ressure liquid chromatography–<?tcaps=1>m<?tcaps=0>ass spectrometry
Author(s) -
Sturm Sonja,
Stuppner Hermann
Publication year - 2000
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(200003/04)11:2<121::aid-pca493>3.0.co;2-9
Subject(s) - chemistry , chromatography , scrophulariaceae , cucurbitaceae , high performance liquid chromatography , citrullus colocynthis , atmospheric pressure chemical ionization , chromatography detector , electrospray ionization , mass spectrometry , traditional medicine , chemical ionization , botany , organic chemistry , ionization , medicine , ion , biology
HPLC–MS (electrospray ionization or atmospheric pressure chemical ionization) was applied to the analysis of cucurbitacins in medicinal plants. High molecular weight plant constituents were separated from cucurbitacins by gel chromatography of the crude methanolic extracts. HPLC was performed on a Zorbax SB‐C‐18 column using a 0.01% trifluoroacetic acid:acetonitrile gradient and diode array detection. The method described was sensitive, and its applicability was demonstrated through the determination of cucurbitacins in a number of medicinal plants such as Citrullus colocynthis (Cucurbitaceae), Bryonia cretica ssp. dioica (Cucurbitaceae), Gratiola officinalis (Scrophulariaceae), Picrorhiza kurroa (Scrophulariaceae) and Iberis umbellata (Brassicaceae). Copyright © 2000 John Wiley & Sons, Ltd.