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Evaluation of different detection modes for the analysis of procyanidins in leaves and flowers of Crataegus spp. Part I. Diode array and electrochemical detection
Author(s) -
Rohr Gabriela E.,
Meier Beat,
Sticher Otto
Publication year - 2000
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(200003/04)11:2<106::aid-pca496>3.0.co;2-o
Subject(s) - chemistry , absorbance , detection limit , chromatography , electrochemistry , analytical chemistry (journal) , monomer , electrode , selectivity , quantitative analysis (chemistry) , organic chemistry , polymer , catalysis
Diode array and single electrode electrochemical detection were evaluated for their use in the analysis of (−)‐epicatechin and oligomeric procyanidins in reference solutions and extracts of leaves and flowers of Crataegus spp. Linearity of regression curves was good for both detection modes. Diode array detection was found to be more selective but less sensitive than electrochemical detection. The absorbance ratio 220/280 nm gives information on the degree of polymerization in reference solutions. In the presence of matrix no additional structural information can be obtained from the absorbance ratios due to high standard deviations. Quantitative evaluation in terms of monomer equivalents using electrochemical detection leads to an underestimation of the contents of procyanidin dimers, but it is applicable using detection at 280 nm. In view of the better selectivity and the easier handling, diode array detection is recommended for the analysis of procyanidins in complex matrices. Copyright © 2000 John Wiley & Sons, Ltd.