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Non‐aqueous capillary electrophoresis for the simultaneous analysis of solasodine and solasonine
Author(s) -
Kreft Samo,
Z̆el Jana,
Pukl Marko,
Umek Andrej,
S̆trukelj Borut
Publication year - 2000
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(200001/02)11:1<37::aid-pca490>3.0.co;2-5
Subject(s) - solasodine , chemistry , chromatography , capillary electrophoresis , aqueous solution , sodium hydroxide , detection limit , acetic acid , sodium , organic chemistry , botany , solanum , biology
A capillary electrophoresis method for the simultaneous determination of the steroidal alkaloid solasodine and the derived glycoside solasonine in plant samples has been developed. Because of the low solubility of solasodine in water and in water:ethanol solutions, a non‐aqueous buffer (600 m M acetic acid, 10 m M sodium hydroxide in methanol) was utilized. The signal was measured at 200 nm and the detection limit of solasodine was 8 mg/L. The method is fast, simple and precise, and good separations were obtained with the crude plant extracts. The influence of sodium and chloride ions in the sample matrix on the performance of the method is discussed. Copyright © 2000 John Wiley & Sons, Ltd.