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High‐Performance Thin‐Layer Chromatographic analysis of hepatoprotective diterpenoids from Andrographis paniculata
Author(s) -
Saxena Sudhanshu,
Jain Dharam C.,
Gupta Madan M.,
Bhakuni Rajendra S.,
Mishra Hari O.,
Sharma Ram P.
Publication year - 2000
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(200001/02)11:1<34::aid-pca487>3.0.co;2-v
Subject(s) - andrographolide , andrographis paniculata , chemistry , chromatography , silica gel , thin layer chromatography , thin layer , extraction (chemistry) , organic chemistry , layer (electronics) , medicine , alternative medicine , pathology
A rapid and simple high‐performance thin‐layer chromatographic (HPTLC) method has been developed for the simultaneous quantitative estimation of the biologically active diterpenoids, 14‐deoxy‐11,12‐didehydroandrographolide, andrographolide, neoandrographolide and andrographiside in Andrographis paniculata . The assay combines the isolation and separation of andrographolide derivatives on silica gel 60 F 254 HPTLC plates with spot visualization and scanning at 540 nm. Methanol was found to be the most appropriate solvent for the exhaustive extraction of andrographolide derivatives. Copyright © 2000 John Wiley & Sons, Ltd.