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Qualitative analysis of indigo precursors from woad by HPLC and HPLC‐MS
Author(s) -
Gilbert nee Stoker K. G.,
Hill D. J.,
Crespo C.,
Mas A.,
Lewis M.,
Rudolph B.,
Cooke D. T.
Publication year - 2000
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(200001/02)11:1<18::aid-pca483>3.0.co;2-x
Subject(s) - chemistry , high performance liquid chromatography , chromatography , qualitative analysis , mass spectrometry , botany , qualitative research , social science , sociology , biology
A qualitative method has been devised to analyse the indigo precursors in leaf extracts of woad ( Isatis spp.) using HPLC coupled to an evaporative light scattering detector and confirmed using HPLC coupled, on line, to a particle beam mass spectrometer. Using this technique, indican has been positively identified in shoot extracts of European ( I. tinctoria ) and Chinese ( I. indigotica ) woad. Greater amounts of indican were found in the Chinese compared with the European woad. Copyright © 2000 John Wiley & Sons, Ltd.