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Ion‐pair HPLC determination of hydroxycinnamic acid monoconjugates of putrescine, spermidine and spermine
Author(s) -
Panagabko Candace,
Chenier Deborah,
FixonOwoo Solomon,
Atkinson Jeffrey K.
Publication year - 2000
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(200001/02)11:1<11::aid-pca479>3.0.co;2-0
Subject(s) - chemistry , putrescine , spermidine , hydroxycinnamic acid , spermine , chromatography , reagent , polyamine , extraction (chemistry) , high performance liquid chromatography , methanol , solid phase extraction , sodium , organic chemistry , biochemistry , enzyme , antioxidant
The terminal hydroxycinnamic acid monoconjugates of putrescine, spermidine and spermine were resolved and quantified in extracts of plant tissues using sodium hexanesulphonate as an ion‐pair reagent and a double methanol gradient. Extracts were prepared from acidic methanol:water (80:20) that had been back‐extracted with hexane, and fractionated on C‐18 solid phase extraction and carboxymethyl cation exchange cartridges. The chromatographic conditions were capable of separating N 1 ‐ and N 8 ‐coumaroyl and feruloyl spermidine, which differ only by the disposition of the secondary amine in the polyamine side chain. Reliable detection limits for most compounds were less than 5 µg/mL (approximately 10 −5 M in the plant extract) and extraction efficiencies were 91% as determined using the novel internal standard, N 1 ‐coumaroyl hexanediamine. Copyright © 2000 John Wiley & Sons, Ltd.