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LC/ES‐MS analysis of triterpene glycosides: rapid estimation of the saponin content of dried berries of Phytolacca dodecandra
Author(s) -
Perret C.,
Wolfender J.L.,
Hostettmann K.
Publication year - 1999
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199909/10)10:5<272::aid-pca470>3.0.co;2-z
Subject(s) - chemistry , saponin , triterpene , chromatography , glycoside , electrospray ionization , aglycone , tandem mass spectrometry , fragmentation (computing) , electrospray , mass spectrometry , stereochemistry , medicine , alternative medicine , pathology , computer science , operating system
A liquid chromatographic/mass spectrometric (MS) method involving electrospray ionization together with up‐front collision‐induced dissociation, tandem MS or MS n fragmentation has been developed for rapid on‐line characterization of triterpene glycosides. Sensitive detection was obtained by post‐column addition of alkaline buffer, providing intense deprotonated molecular ions. The experimental method has provided information on the sugar sequence and aglycone of each saponin within a crude plant extract. The method has been applied for the rapid estimation of saponin content in methanolic and aqueous extracts of dried berries of Phytolacca dodecandra (Phytolaccaceae). Copyright © 1999 John Wiley & Sons, Ltd.