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Simultaneous determination of phenolic acids and linear furanocoumarins in fruits of libanotis dolichostyla by solid‐phase extraction and high performance liquid chromatography
Author(s) -
Zgórka Grażyna,
Gowniak Kazimierz
Publication year - 1999
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199909/10)10:5<268::aid-pca467>3.0.co;2-z
Subject(s) - chemistry , chromatography , extraction (chemistry) , acetonitrile , high performance liquid chromatography , solid phase extraction , amine gas treating , organic chemistry
A new, reproducible and simple method combining solid‐phase extraction (SPE) and reversed‐phase high performance liquid chromatography for the simultaneous determination of phenolic acids and linear furanocoumarins in Libanotis dolichostyla fruits has been developed. A preparative separation of the compounds examined was carried out on Baker‐Bond ® octadecyl and quaternary amine SPE‐columns using various concentrations of methanol and acetonitrile in water. High and reproducible recoveries were obtained. The results showed that the concentration of compounds examined was highly dependent on the stage of fruit maturation, confirming the possible role of phenolic acids and furanocoumarins as phytoalexins. Copyright © 1999 John Wiley & Sons, Ltd.