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Rapid detection of polyhydroxyalkaloid mono‐ and diglycosides in crude plant extracts by direct quadrupole ion trap mass spectrometry
Author(s) -
Kite Geoffrey C.,
Porter Elaine A.,
Egan Martin J.,
Simmonds Monique S. J.
Publication year - 1999
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199909/10)10:5<259::aid-pca463>3.0.co;2-8
Subject(s) - chemistry , quadrupole ion trap , mass spectrometry , ion trap , chromatography , trap (plumbing) , quadrupole time of flight , ion mobility spectrometry , ion , analytical chemistry (journal) , tandem mass spectrometry , organic chemistry , environmental engineering , engineering
Direct MS analysis, utilizing first‐order MS and subsequent MS 2 and MS 3 product ion analyses, is shown to provide a rapid means of characterizing polyhydroxyalkaloid glycosides and aglycones in aqueous methanol plant extracts that have been crudely purified on ion exchange resin. Analysis of species known to synthesize polyhydroxyalkaloids resulted in the discovery of the first diglycosides of these compounds. These were detected in Omphalea diandra and Syzygium oleosum . A monoglycoside was also detected as a minor component in Castanospermum australe . Copyright © 1999 John Wiley & Sons, Ltd.