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Identification of Isoquinoline alkaloids in crude extracts by high temperature gas chromatography‐mass spectrometry
Author(s) -
Pereira Alberto S.,
Amaral Ana Claudia F.,
Barnes Roderick A.,
Cardoso Jari N.,
Aquino Neto Francisco R.
Publication year - 1999
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199909/10)10:5<254::aid-pca462>3.0.co;2-q
Subject(s) - chemistry , isoquinoline , chromatography , mass spectrometry , fractionation , gas chromatography , alkaloid , gas chromatography–mass spectrometry , phytochemical , croton , organic chemistry , biochemistry
Crude ethanol extracts of leaves of Croton (Euphorbiaceae) species ( C. hemiargyreus var. gymnodiscus, C. hemiargyreus var. hemiargyreus and C. echinocarpus ) were analysed using on‐column injection high temperature gas chromatography coupled with mass spectrometry. Several isoquinoline alkaloids were identified, including isocorydine, corydine, glaucine and laurelliptine as the principal constituents. The cold on‐column technique proved to be appropriate for injection of these compounds. The technique is shown to be a valuable tool and an alternative technique to classical phytochemical procedures permitting the fast analysis of alkaloid mixtures without the need for (extensive) fractionation and derivatisation. Copyright © 1999 John Wiley & Sons, Ltd.