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Reverse‐phase high performance liquid chromatography of asiaticoside in Centella asiatica
Author(s) -
Verma R. K.,
Bhartariya K. G.,
Gupta M. M.,
Kumar Sushil
Publication year - 1999
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199907/08)10:4<191::aid-pca456>3.0.co;2-r
Subject(s) - centella , chemistry , chromatography , trifluoroacetic acid , elution , methanol , high performance liquid chromatography , silane , organic chemistry , traditional medicine , medicine
A rapid and simple reverse‐phase high performance liquid chromatographic method has been developed for the quantitative determination of asiaticoside, a pharmacologically active constituent from Centella asiatica . Using an octadecyl silane‐packed column eluted with water (containing 1% trifluoroacetic acid):methanol (30:70, v/v), asiaticoside was well resolved from other constituents. These optimized analytical conditions allowed the determination of asiaticoside with a recovery of 97%. Copyright © 1999 John Wiley & Sons, Ltd.