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Pine needle abortion in cattle: analysis of isocupressic acid in North American gymnosperms
Author(s) -
Gardner Dale R.,
James Lynn F.
Publication year - 1999
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199905/06)10:3<132::aid-pca444>3.0.co;2-b
Subject(s) - chemistry , labdane , phytochemical , resin acid , acid hydrolysis , shrub , pinus <genus> , trimethylsilyl , chromatography , botany , hydrolysis , terpenoid , organic chemistry , biochemistry , biology
Isocupressic acid is a labdane resin acid found in Pinus ponderosa needles and causes abortion in late term pregnant cattle. A chemical survey was conducted to determine the phytochemical distribution of isocupressic acid in selected North American shrub and tree species with possible association with cattle abortions. A method of analysis for the evaluation of total available isocupressic acid (including hydrolysed acetyl and succinyl derivatives) was developed based on a base/acid extraction of dry, ground plant material, followed by detection and quantitative determination using capillary gas chromatography of the di‐(trimethylsilyl)‐derivative. Samples from 23 different species were collected throughout the western and southern regions of the United States and analysed. Isocupressic acid was detected in 15 species, and significant concentrations (>0.5% dry weight) were found in Pinus ponderosa, P. jeffreyi, P. contorta, Juniperus scopulorum, and J. communis. Copyright © 1999 John Wiley & Sons, Ltd.