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Electrospray liquid chromatography–mass spectrometry of the leaf extract of Rhamnus prinoides
Author(s) -
Nindi M. M.,
Kgarebe B. V.,
Wolfender J.L.,
Abegaz B. M.
Publication year - 1999
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199903/04)10:2<69::aid-pca447>3.0.co;2-4
Subject(s) - chemistry , chromatography , electrospray , electrospray ionization , mass spectrometry , anthraquinones , electrospray mass spectrometry , liquid chromatography–mass spectrometry , botany , biology
Three naphthalenic derivatives, four flavonoids and two of the four anthraquinones previously isolated from the leaves of Rhamnus prinoides were successfully ionised under electrospray ionisation conditions. These compounds were subsequently detected in the leaf extract of the plant using electrospray liquid chromatography–mass spectrometry (LC–ESI–MS). The potential of LC–ESI–MS for screening a leaf extract, with the focus on the suitability of the method for assaying the bitter principle, geshoidin, of this commercially important plant, is presented. Copyright © 1999 John Wiley & Sons, Ltd.