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Droplet counter‐current chromatography of indole alkaloids from Tabernaemontana hilariana
Author(s) -
Cardoso Claudia Andrea Lima,
Vilegas Wagner
Publication year - 1999
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199903/04)10:2<60::aid-pca446>3.0.co;2-y
Subject(s) - chemistry , apocynaceae , chromatography , indole test , thin layer chromatography , indole alkaloid , alkaloid , countercurrent chromatography , ethyl acetate , gas chromatography , hexane , fraction (chemistry) , mass spectrometry , ethanol , column chromatography , organic chemistry , traditional medicine , medicine
This paper reports the separation of the indole alkaloids from the benzene extract of the root barks of Tabernaemontana hilariana (Apocynaceae). The crude alkaloid fraction was fractionated by droplet counter‐current chromatography using a low polarity mixture (hexane:ethyl acetate:ethanol:water). Nine indole alkaloids (3‐hydroxycoronaridine, coronaridine, voacangine, 3‐(2‐oxopropyl) coronaridine, voacangine hydroxyindolenine, ibogamine, voacangine pseudoindoxyl, coronaridine pseudoindoxyl and tabernanthine) were identified using thin layer chromatography, gas chromatography coupled with mass spectrometry and nuclear magnetic resonance spectroscopy. Copyright © 1999 John Wiley & Sons, Ltd.