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Separation and identification of organic acids in root exudates of Lupinus luteus by capillary zone electrophoresis
Author(s) -
Barbas C.,
García J. A. Lucas,
Mañero F. J. Gutiérrez
Publication year - 1999
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199903/04)10:2<55::aid-pca437>3.0.co;2-i
Subject(s) - chemistry , capillary electrophoresis , chromatography , oxalic acid , electrophoresis , cationic polymerization , organic acid , citric acid , fumaric acid , lactic acid , organic chemistry , bacteria , biology , genetics
The separation and identification of a mixture of organic acids by capillary electrophoresis, with direct detection by UV absorption at a wavelength of 200 nm is reported. A solution containing the following organic acids was studied: oxalic, formic, fumaric, malic, succinic, citric, acetic and lactic acid. Separation was achieved using a fused‐silica capillary filled with phosphate buffer (200 m M ; pH 6) containing N ‐acetyl‐ N,N,N ‐trimethylammonium bromide (0.5 m M ) as cationic surfactant, necessary to modify the electrosmotic flow. The composition of organic acids in exudates of Lupinus luteus was determined by this method. Copyright © 1999 John Wiley & Sons, Ltd.