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High performance liquid chromatographic quantification of salvinorin a from tissues of salvia divinorum epling & játiva‐m
Author(s) -
Gruber John W.,
Siebert Daniel J.,
Marderosian Ara H. Der,
Hock Rick S.
Publication year - 1999
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199901/02)10:1<22::aid-pca428>3.0.co;2-0
Subject(s) - chemistry , chromatography , diterpene , gradient elution , high performance liquid chromatography , organic chemistry
A reversed‐phase high performance liquid chromatographic method for the determination of salvinorin A, a psychotropic diterpene isolated from the Mexican sage Salvia divinorum , has been developed. Extracts from several plant collections were examined on a C‐18 column with UV detection and isocratic elution with acetonitrile: water (45:55). This assay allowed quantification of salvinorin A in extracts of leaves and stems of S. divinorum and has also been applied to the screening of related species for the production of salvinorin A. Levels of salvinorin A in leaves range from 0.89 to 3.70 mg/g dry weight. Copyright © 1999 John Wiley & Sons, Ltd.