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Quantitative determination of maitenin and 22β‐hydroxymaitenin in callus of Maytenus aquifolium (celastraceae) by reverse phase high performance liquid chromatography
Author(s) -
Corsino Joaquim,
Alécio Alberto Camilo,
Ribeiro Maria Lúcia,
Furlan Maysa,
Pereira Ana Maria S.,
Duarte Ines Batista,
França Suzelei Castro
Publication year - 1998
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199809/10)9:5<245::aid-pca413>3.0.co;2-t
Subject(s) - chemistry , callus , chromatography , high performance liquid chromatography , triterpene , celastraceae , elution , stereochemistry , botany , medicine , alternative medicine , pathology , biology
Explants of Maytenus aquifolium were induced to form callus and, subsequently, suspension cultures. The isolation of natural products from callus led to the identification of the cytotoxic triterpene quinonemethides, maitenin ( 1 ) and 22β‐hydroxymaitenin ( 2 ). A rapid, sensitive and reliable reversed‐phase high‐performance liquid chromatography method was developed using a C 18 column and isocratic elution for the determination of 1 and 2 . The elaborated method gave well‐resolved peaks for these compounds with good detection response and linearity in the range of 0.08–72.0 μg. The quantification of 1 and 2 was performed by an external standard method. © 1998 John Wiley & Sons, Ltd.