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Determination of atropine and scopolamine by proton nuclear magnetic resonance spectroscopy
Author(s) -
Naqvi A. A.,
Mandal S.,
Verma R. K.
Publication year - 1998
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199807/08)9:4<168::aid-pca408>3.0.co;2-q
Subject(s) - chemistry , atropine , proton magnetic resonance , proton , nuclear magnetic resonance , scopolamine , spectroscopy , nuclear magnetic resonance spectroscopy , singlet state , analytical chemistry (journal) , chromatography , nuclear physics , organic chemistry , medicine , physics , quantum mechanics , anesthesia , excited state
A simple, accurate and specific proton nuclear magnetic resonance spectrometric method is described for the quantitative determination of atropine and scopolamine in plants. The method is based on a comparison of the integrated peak areas of the N‐CH 3 protons of both compounds (centred at 2.15 and 2.5 ppm) with that of the sharp singlet of 1, 4‐dioxane (positioned at 3.5 ppm) which is used as the internal standard. © 1998 John Wiley & Sons, Ltd.

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