Premium
A simplified method for the determination of taxanes in yew needles by reversed‐phase (C 18 ) high pressure liquid chromatography
Author(s) -
van Rozendaal Erik L. M.,
Lelyveld Gerrit P.,
van Beek Teris A.
Publication year - 1997
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199711/12)8:6<286::aid-pca374>3.0.co;2-g
Subject(s) - chemistry , chromatography , column chromatography , acetic acid , high performance liquid chromatography , reproducibility , acetonitrile , cinnamic acid , detection limit , organic chemistry
An analytical procedure for the large‐scale qualitative and quantitative screening of paclitaxel (Taxol ® ) and related neural taxanes in yew needles has been developed. The needles were selectively extracted with ethanol:water:acetic acid (80:19:1) and the resulting extract was purified from chlorophyll, 3,5‐dimethyoxy phenol, taxine alkaloids and cinnamoyl taxanes, by means of small‐scale partition chromatography on Extrulet ® . After concentration, the taxanes were determined by high pressure chromatography on a C 18 column with acetonitrile;water as eluent and ultra violet detection at 227 and 280 nm. Cinnamic acid was used as an internal standard. Various aspects such as extraction efficiency, recovery, peak purity, detection limits, robustness and reproducibility of the method have been evaluated. The method is very labour efficient with only 20 min of manpower input per sample. © 1997 John Wiley & Sons, Ltd.