Premium
Supercritical fluid carbon dioxide extraction of annatto seeds and quantification of trans ‐bixin by high pressure liquid chromatography
Author(s) -
Anderson Simon G.,
Nair Muraleedharan G.,
Chandra Amitabh,
Morrison Errol
Publication year - 1997
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199709/10)8:5<247::aid-pca361>3.0.co;2-v
Subject(s) - chemistry , supercritical carbon dioxide , extraction (chemistry) , chromatography , acetonitrile , carbon dioxide , trifluoroacetic acid , supercritical fluid extraction , high performance liquid chromatography , supercritical fluid chromatography , pigment , organic chemistry
Bixin, a pigment used for food colouring is known to have a hyperglycemic effect. It was extracted using supercritical carbon dioxide, from the seeds of annatto ( Bixa orellana L.). The major pigment in annatto, trans ‐bixin, was quantified by high pressure liquid chromatography. A combination of static and dynamic modes of extraction with carbon dioxide containing acetonitrile (0.05% trifluoroacetic acid) as modifier at 60.62 MPa and 40°C was found to the best set of conditions studied and afforded 2.70 mg bixin/g dry weight of the annatto seeds. © 1997 John Wiley & Sons, Ltd.