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Establishing styrylpyrone synthase activity in cell free extracts obtained from gametophytes of Equisetum arvense L. by high performance liquid chromatography–tandem mass spectrometry
Author(s) -
Herderich Markus,
Beckert Cornelia,
Veit Markus
Publication year - 1997
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199707)8:4<194::aid-pca357>3.0.co;2-6
Subject(s) - chemistry , polyketide synthase , tandem mass spectrometry , chromatography , atp synthase , high performance liquid chromatography , mass spectrometry , atmospheric pressure chemical ionization , liquid chromatography–mass spectrometry , chemical ionization , biochemistry , polyketide , biosynthesis , enzyme , ionization , organic chemistry , ion
Styrylpyrone synthase is a polyketide synthase, catalyzing the formation of styrylpyrones from hydroxy‐ cinnamoyl‐CoA precursors and malonyl‐CoA. After incubation of cell free extracts obtained from Equisetum arvense L. gametophytes with p ‐coumaroyl‐CoA or caffeoyl‐CoA, high performance liquid chromatography atmospheric pressure chemical ionization‐tandem mass spectrometry (HPLC‐APCI‐MS) led to the identification of bisnoryangonin and hispidin, respectively, as metabolites. These findings enabled the development of an assay for styrylpyrone synthase activity based on HPLC analysis of the reaction products. The technique could also be useful for product identification in related polyketide synthase reactions. © 1997 John Wiley & Sons, Ltd.