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A general method for the dereplication of flavonoid glycosides utilizing high performance liquid chromatography/mass spectrometric analysis
Author(s) -
Constant Howard L.,
Slowing Karla,
Graham James G.,
Pezzuto John M.,
Cordell Geoffrey A.,
Beecher Christopher W. W.
Publication year - 1997
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/(sici)1099-1565(199707)8:4<176::aid-pca351>3.0.co;2-k
Subject(s) - chemistry , aglycone , flavonoid , chromatography , glycoside , mass spectrometry , electrospray , electrospray ionization , organic chemistry , antioxidant
Using high performance liquid chromatography–electrospray mass spectrometry a method was developed to separate flavonoids and flavonoid glycosides and to obtain molecular weight information. Collision‐induced dissociation was then used to obtain the molecular masses of the flavonoid aglycones. Thus, the mass of a flavonoid glycoside and its aglycone can now be determined in a mixture, and it is not necessary to isolate the components. An aqueous extract of Eugenia jambos L. was used to demonstrate the utility of the technique in analysing extracts for flavonoid glycosides. © 1997 John Wiley & Sons, Ltd.